Human OX40 Protein, hFc-His tag

• Catalog Number: orb689383
• Category: Proteins
• Description: Recombinant human OX40 protein with C-terminal human Fc and 6×His tag
• Reactivity: Human
• Tag: C-Human Fc and 6×His Tag
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• MW: The protein has a predicted molecular mass of 47.2 kDa after removal of the signal peptide.
• Target: OX40
• UniProt ID: P43489
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Alternative names: TNFRSF4;OX40;CD134;OX40L receptor;ACT35;TXGP1L
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human CD27 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD27, mFc-His tagged protein (orb689374) can bind Anti-CD27 Neutralizing antibody orb689323 in a linear range of 0.64-16.0 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD27, mFc-His tagged protein (orb689374) can bind Human CD70, hFc-His tagged protein orb689468 in a linear range of 3.2-80 ng/ml.

Flow cytometry analysis with 15 μg/mL Human CD27 Protein, mFc-His tag (orb689374) on Expi293 cells transfected with human CD70 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).