Recombinant Human Noggin

Catalog Number: orb1906230

Catalog Number	orb1906230
Category	Proteins
Description	Recombinant Human Noggin
Form/Appearance	Lyophilized from a 0.2 um filtered concentrated solution in PBS.
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Protein Sequence	MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC
UniProt ID	Q13253
MW	Approximately 46.3 kDa, non-disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.
Expression System	Escherichia coli
Endotoxins	Less than 1 EU/ug of rHuNoggin as determined by LAL method.
Source	Escherichia coli
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 3.0 ng/ml, corresponding to a specific activity of > 3.3 �� 105 IU/mg in the presence of 5 ng/ml rHuBMP-4.
Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Note	For research use only

Purity:	> 95 % by SDS-PAGE and HPLC analyses.
MW:	Approximately 23.0 kDa on SDS-PAGE under reducing conditions, containing 205 amino acids, and a molecular mass about 47.9 kDa homodimer under non-reducing conditions (Molecular size on SDS-PAGE will appear at approximately 50-80 kDa).
Source:	Insect Cell

Sizes Available: 5 μg, 100 μg, 500 μg

Purity:	Greater than 85% as determined by SDS-PAGE.
MW:	58.2 kDa
Source:	E.coli

Purity:	Greater than 95% as determined by reducing SDS-PAGE.
MW:	50.2 KDa
Source:	Mammalian

Purity:	> 95% as determined by Tris-Bis PAGE
MW:	Due to glycosylation, the protein migrates to 30-36 kDa based on Tris-Bis PAGE result.