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Catalog Number | orb1096190 |
---|---|
Category | Proteins |
Description | Recombinant Human Noggin(NOG) |
Tag | N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged |
Form/Appearance | Liquid or Lyophilized powder |
Purity | Greater than 85% as determined by SDS-PAGE. |
MW | 58.2 kDa |
UniProt ID | Q13253 |
Protein Sequence | QHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC |
Protein Length | Full Length of Mature Protein |
Source | E.coli |
Expression System | Expression Region: 28-232aa. Protein Length: Full Length of Mature Protein |
Endotoxins | Not test. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
Greater than 95% as determined by reducing SDS-PAGE. | |
50.2 KDa | |
Mammalian |
ELISA | |
> 98% as determined by SDS-PAGE. Ni-NTA chromatography. | |
Escherichia coli |
> 95% by Tris-Bis PAGE | |
KMP2049, Recombinant Human Noggin Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln28-Cys232) of Human Noggin fused with no tag . |
> 85% by SDS-PAGE. | |
KMP1354, Recombinant human Noggin Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Gln28-Cys232) of human Noggin (Accession #NP_005441.1) fused with a 6×His Tag at the C-terminus. |
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