Recombinant Human Leukemia Inhibitory Factor

SKU: orb1906309

Description

Recombinant Human Leukemia Inhibitory Factor

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x107 IU/mg.
• Molecular Weight: Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
• Protein Sequence: SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
• Purity: > 98 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 1 EU/ug of rHuLIF as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Disclaimer: For research use only

Alternative Names

Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor, MLPLI

Quick Database Links

UniProt

P15018