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Recombinant Human Leukemia inhibitory factor (LIF) (Active)

Catalog Number: orb1095883

DispatchUsually dispatched within 5-10 working days
$ 280.00
Catalog Numberorb1095883
CategoryProteins
DescriptionRecombinant Human Leukemia inhibitory factor(LIF) (Active)
TagC-terminal hFc-tagged
Form/AppearanceLyophilized powder
PurityGreater than 90% as determined by SDS-PAGE.
MW48.6 kDa
UniProt IDP15018
Protein SequenceSPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF
Protein LengthFull Length of Mature Protein
SourceMammalian cell
Biological OriginHomo sapiens (Human)
Biological ActivityMeasured by its binding ability in a functional ELISA. Immobilized human LIF at 2 μg/ml can bind human LIFR, the EC50 is 22.58-30.24 ng/ml.
Expression Region23-202aa
EndotoxinsLess than 1.0 EU/ug as determined by LAL method.
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Buffer/PreservativesLyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
NoteFor research use only
Application notesWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Expiration Date6 months from date of receipt.
Recombinant Human Leukemia inhibitory factor (LIF) (Active)

Recombinant Human Leukemia inhibitory factor (LIF) (Active)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

  • Human LIF protein [orb594754]

    Greater than 95% as determined by SDS-PAGE.

    19.7 kDa

    E.coli

    1 mg, 500 μg, 10 μg, 50 μg
  • Murine Oncostatin M protein [orb107791]

    HPLC,  SDS-PAGE

    Unconjugated

    > 96 % by SDS-PAGE and HPLC analyses

    20.3 kDa

    10 μg, 100 μg, 500 μg
  • Recombinant Human Leukemia inhibitory factor (LIF) (Active) [orb2657908]

    Greater than 95% as determined by SDS-PAGE.

    19.7 kDa

    Mammalian cell

    10 μg, 50 μg, 1 mg
  • RecombinantOSM,Rat [orb1494781]

    > 95% as analyzed by SDS-PAGE and HPLC.

    24.5 kDa, observed by reducing SDS-PAGE.

    Escherichia coli.

    50 μg, 10 μg, 1 mg
  • RecombinantOSM,Mouse [orb1494782]

    > 95% as analyzed by SDS-PAGE and HPLC.

    20.5 kDa, observed by reducing SDS-PAGE.

    Escherichia coli.

    50 μg, 10 μg, 1 mg