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Catalog Number | orb623225 |
---|---|
Category | Proteins |
Description | Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. Human IL-6 Recombinant Protein is purified interleukin-6 produced in yeast. |
Form/Appearance | Lyophilized |
MW | 20.8 kDa |
Protein Sequence | VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENN LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKV LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQM (183) |
Source | Yeast |
Storage | -20°C |
Note | For research use only |
Application notes | The Mouse IL-34 protein can be used in cell culture, as an IL-34 ELISA Standard, and as a Western Blot Control. |
Expiration Date | 6 months from date of receipt. |
> 96% as determined by SDS-PAGE and HPLC. | |
20.7 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
20.9 kDa | |
E.coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 47.9 kDa after removal of the signal peptide. The apparent molecular mass of mIL6-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 94% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 65-78 kDa based on Tris-Bis PAGE result. |
> 90% as determined by SDS-PAGE method | |
20.8 kDa |