Recombinant Human IL-17A Protein

SKU: orb2994915

Description

Recombinant Human Interleukin-17A is produced by our Mammalian expression system and the target gene encoding Gly24-Ala155 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human IL-17RA-Fc(Cat#orb2994535) at 2μg/ml (100 μl/well)can bind Human IL-17A-His(Cat#orb2994915).The ED50 of Human IL-17A-His(Cat#orb2994915) is 0.49 ng/ml. Immobilized Human IL-17RA-His(Cat#orb2994076) at 2μg/ml (100 μl/well)can bind Human IL-17A-His(Cat#orb2994915).*: Biotinylated by NHS-biotin prior to testing.The ED50 of Human IL-17A-His(Cat#orb2994915) is 7.39 ng/ml.. BLI: Loaded Human IL-17A Antibody(9E6) (Cat#DA055) on Pro-A Biosensor, can bind Recombinant Human IL-17A (C-6His) (Cat#orb2994915) with an affinity constant of 12.7 pM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human IL-17RA-Fc(Cat#orb2993676) on Pro A Biosensor, can bind Human IL-17A-His(Cat#orb2994915) with an affinity constant of 0.003 nM as determined in BLI assay.. Cell Based Assay: Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1-10 ng/ml.
• Tag: C-6His
• Molecular Weight: Predicted: 15.9 KDa. Observed: 15-22 KDa, reducing conditions
• Expression Region: Gly24-Ala155
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
• Endotoxins: < 0.01 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-17A; IL-17; IL-17A; Cytotoxic T-Lymphocyte-Associated Antigen 8; CTLA-8; IL17A; CTLA8; IL17

Quick Database Links

UniProt

Q16552