Recombinant Human HER2 Protein

SKU: orb2993972

Description

Recombinant Human Receptor Tyrosine-protein Kinase ErbB-2 is produced by our Mammalian expression system and the target gene encoding Thr23-Thr652 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Anti-Human EGFR Antibody (AMI_bio, Research Grade)(Cat#orb2992597) at 1μg/ml (100 μl/well) can bind Recombinant Human EGFR (C-Fc)(Cat#orb2994534)*. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human EGFR (C-Fc)(Cat#orb2994534) is 22.33ng/ml.(Regularly tested).
• Tag: C-Fc
• Molecular Weight: Predicted: 96.5 KDa. Observed: 120-145 KDa, reducing conditions
• Expression Region: Thr23-Thr652
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Tyrosine kinase-type cell surface receptor HER2; ERBB2; MLN19; NGL; TKR1

Quick Database Links

UniProt

P04626