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Description
Research Area
Signal Transduction
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human HER2(Cat#orb2994263) at 1μg/ml (100 μl/well) can bind Anti-Human HER2（Trastuzumab）(Cat#Norb2993550).The EC50 of Anti-Human HER2（Trastuzumab）(Cat#Norb2993550) is 3.97 ng/ml.(Regularly tested). BLI: Loaded Anti-Human HER2 mAb-Fc(Cat#orb2993152) on Protein A Biosensor, can bind Human HER2-His(Cat#orb2994263) with an affinity constant of 1.36 nM as determined in BLI assay. |
| Tag | C-6His |
| Expression Region | Thr23-Thr652 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 70.2 KDa. Observed: 90-120 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS,pH7.4 |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu;Tyrosine kinase-type cell surface receptor HER2; ERBB2; MLN19; NGL; TKR1
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Quick Database Links
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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