Recombinant Human HER2 Protein

SKU: orb2994263

Description

Recombinant Human Receptor Tyrosine-protein Kinase ErbB-2 is produced by our Mammalian expression system and the target gene encoding Thr23-Thr652 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human HER2(Cat#orb2994263) at 1μg/ml (100 μl/well) can bind Anti-Human HER2（Trastuzumab）(Cat#Norb2993550).The EC50 of Anti-Human HER2（Trastuzumab）(Cat#Norb2993550) is 3.97 ng/ml.(Regularly tested). BLI: Loaded Anti-Human HER2 mAb-Fc(Cat#orb2993152) on Protein A Biosensor, can bind Human HER2-His(Cat#orb2994263) with an affinity constant of 1.36 nM as determined in BLI assay.
• Tag: C-6His
• Molecular Weight: Predicted: 70.2 KDa. Observed: 90-120 KDa, reducing conditions
• Expression Region: Thr23-Thr652
• Purity: SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS,pH7.4
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu;Tyrosine kinase-type cell surface receptor HER2; ERBB2; MLN19; NGL; TKR1

Quick Database Links

UniProt

P04626