Recombinant Human Fibroblast Growth Factor 23

SKU: orb1906281

Description

Recombinant Human Fibroblast Growth Factor 23

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 x103 IU/mg in the presence of 0.3 μg/ml of rMuKlotho and 10 μg/ml of heparin.
• Molecular Weight: Approximately 25.3 kDa, a single non-glycosylated polypeptide chain containing 227 amino acids.
• Protein Sequence: YPNASPLLGS SWGGLIHLYT ATARNSYHLQ IHKNGHVDGA PHQTIYSALM IRSEDAGFVV ITGVMSRRYL CMDFRGNIFG SHYFDPENCR FQHQTLENGY DVYHSPQYHF LVSLGRAKRA FLPGMNPPPY SQFLSRRNEI PLIHFNTPIP RRHTRSAEDD SERDPLNVLK PRARMTPAPA SCSQELPSAE DNSPMASDPL GVVRGGRVNT HAGGTGPEGC RPFAKFI
• Purity: > 95 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 1 EU/ug of rHuFGF-23 as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Disclaimer: For research use only

Quick Database Links

UniProt

Q9GZV9