Human EGFR Isoform vIII Protein

SKU: orb2994408

Description

Recombinant Human Epidermal Growth Factor Receptor/Receptor Tyrosine Protein Kinase ErbB1 is produced by our Mammalian expression system and the target gene encoding Leu25-Ser378 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Anti-Human EGFR mAb(Cat#orb2993008) on Protein A Biosensor, can bind Human EGFR vIII-His(Cat#orb2994408) with an affinity constant of 2.55 nM as determined in BLI assay. (Regularly tested)
• Tag: C-6His
• Molecular Weight: Predicted: 39.7 KDa. Observed: 61-75 Kda, reducing conditions
• Expression Region: Leu25-Ser378
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Epidermal growth factor receptor; EGFR; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR

Quick Database Links

UniProt

NP_001333870