Biotinylated Human EGFR Isoform vIII Protein

• Catalog Number: orb2993951
• Category: Proteins
• Description: Biotinylated Recombinant Human Epidermal Growth Factor Receptor/Receptor Tyrosine Protein Kinase ErbB1 is produced by our Mammalian expression system and the target gene encoding Leu25-Ser378 is expressed with a 6His, Avi tag at the C-terminus.
• Conjugation: Biotin
• Tag: C-6His-Avi
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• UniProt ID: NP_001333870
• MW: Predicted: 41.5 KDa. Observed: 60-90 KDa, reducing conditions
• Expression System: Human Cells
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Biological Origin: Human
• Biological Activity: BLI: Loaded Anti-Human EGFR mAb (Cat#orb2993008) on Pro-A Biosensor, can bind Biotinylated Human EGFR vIII (C-6His-Avi) (Cat#orb2993951) with an affinity constant of 3.64 nM as determined in BLI assay. (Regularly tested)
• Expression Region: Leu25-Ser378
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Alternative names: Epidermal growth factor receptor; EGFR; Proto-onco
• Note: For research use only
• Expiration Date: 6 months from date of receipt.