Human EGFR

• Catalog Number: orb2994530
• Category: Proteins
• Description: Recombinant Human Epidermal Growth Factor Receptor is produced by our Mammalian expression system and the target gene encoding Leu25-Ser645 is expressed with a 6His tag at the C-terminus.
• Conjugation: Unconjugated
• Tag: C-6His
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Sucrose, 50mM Nacl, 0.05% Tween80, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• UniProt ID: P00533
• MW: Predicted: 69.6 KDa. Observed: 90-120 KDa, reducing conditions
• Expression System: Human Cells
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Biological Origin: Human
• Biological Activity: BLI: Loaded Anti-Human EGFR mAb-Fc (Cat#orb2993008) on Pro A Biosensor, can bind Recombinant Human EGFR-His (Cat#orb2994530) with an affinity constant of 7.23 nM as determined in BLI assay.
• Expression Region: Leu25-Ser645
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Alternative names: Epidermal growth factor receptor; Proto-oncogene c
• Note: For research use only
• Expiration Date: 6 months from date of receipt.