Human CTLA-4

• Catalog Number: orb2994252
• Category: Proteins
• Description: Recombinant Human Cytotoxic T-lymphocyte Protein 4 is produced by our Mammalian expression system and the target gene encoding Lys36-Asp161 is expressed with a GST tag at the C-terminus.
• Conjugation: Unconjugated
• Tag: C-GST
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• UniProt ID: P16410
• MW: Predicted: 39.2 KDa. Observed: 40-55 KDa, reducing conditions
• Expression System: Human Cells
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Mouse B7-1-Fc (Cat#orb2994397) at 5μg/ml (100 μl/well) can bind Human CTLA-4-GST*(Cat#orb2994252).*: Biotinylated by NHS-biotin prior to testing The ED50 of Recombinant Human CTLA-4-GST (Cat#orb2994252) is 1.17 ug/ml.
• Expression Region: Lys36-Asp161
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Alternative names: Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymp
• Note: For research use only
• Expiration Date: 6 months from date of receipt.