Human CTLA-4

SKU: orb2994189

Description

Recombinant Human Cytotoxic T-lymphocyte Protein 4 is produced by our Mammalian expression system and the target gene encoding Lys36-Asp161 is expressed with a Flag tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Recombinant Human B7-2 (C-Fc) (Cat#orb2992753) on Pro-A Biosensor, can bind Recombinant Human CTLA-4 (C-Flag) (Cat#orb2994189) with an affinity constant of 45.9 nM as determined in BLI assay. (Regularly tested)
• Tag: C-Flag
• Molecular Weight: Predicted: 14.5 KDa. Observed: 20 KDa, reducing conditions
• Expression Region: Lys36-Asp161
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB,50mM NaCl,8% Trehalose,4% Mannitol,0.02% Tween 80,pH6.6
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD152; CTLA4

Quick Database Links

UniProt

P16410