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Catalog Number | orb2658983 |
---|---|
Category | Proteins |
Description | Recombinant Human Ciliary neurotrophic factor (CNTF) |
Tag | N-terminal 10xHis-tagged |
Form/Appearance | Liquid or Lyophilized powder |
Purity | Greater than 95% as determined by SDS-PAGE. |
MW | 29 kDa |
UniProt ID | P26441 |
Protein Sequence | MAFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFAYQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFISSHQTGIPARGSHYIANNKKM |
Protein Length | Full Length |
Source | E.coli |
Biological Origin | Homo sapiens (Human) |
Expression Region | 1-200aa |
Endotoxins | Not test. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
> 97 % by SDS-PAGE and HPLC analyses. | |
Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids. | |
Escherichia coli |
Greater than 95% as determined by reducing SDS-PAGE. | |
22.93 KDa | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
26.1 kDa | |
E.coli |
Greater than 85% as determined by SDS-PAGE. | |
26.8 kDa | |
Baculovirus |
Greater than 85% as determined by SDS-PAGE. | |
27.0 kDa | |
E.coli |