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Recombinant Human Ciliary Neurotrophic Factor
SKU: orb1906246
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Description
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 �� 103 IU/mg. |
| Molecular Weight | Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids. |
| Protein Sequence | MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1 EU/ug of rHuCNTF as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 um filtered concentrated solution in PBS. |
| Disclaimer | For research use only |
Alternative Names
−CNTF
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Predicted: 22.93 KDa. Observed: 25 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg- Item 1 of 1
Recombinant Human Ciliary neurotrophic factor (CNTF) [orb2658983]
Greater than 95% as determined by SDS-PAGE.
29 kDa
E.coli
1 mg, 100 μg, 20 μg
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Recombinant Human Ciliary Neurotrophic Factor (orb1906246)
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