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Catalog Number | orb1906246 |
---|---|
Category | Proteins |
Description | Recombinant Human Ciliary Neurotrophic Factor |
Form/Appearance | Lyophilized from a 0.2 um filtered concentrated solution in PBS. |
Purity | > 97 % by SDS-PAGE and HPLC analyses. |
MW | Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids. |
UniProt ID | P26441 |
Protein Sequence | MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM |
Source | Escherichia coli |
Expression System | Escherichia coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 �� 103 IU/mg. |
Endotoxins | Less than 1 EU/ug of rHuCNTF as determined by LAL method. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Alternative names | CNTF Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Greater than 95% as determined by reducing SDS-PAGE. | |
22.93 KDa | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
26.1 kDa | |
E.coli |
Greater than 85% as determined by SDS-PAGE. | |
26.8 kDa | |
Baculovirus |
Greater than 85% as determined by SDS-PAGE. | |
27.0 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
29 kDa | |
E.coli |