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Human CD47
SKU: orb2994047
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Anti-Human CD47 mAb-Fc(Cat#orb2993150) on AHC Biosensor, can bind Human CD47-mFc(Cat#orb2994047) with an affinity constant of 0.08 nM as determined in BLI assay. |
| Tag | C-mFc |
| Molecular Weight | Predicted: 40.3 KDa. Observed: 48-65 KDa, reducing conditions |
| Expression Region | Gln19-Pro139 |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD47; MER6
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Human CD47 Protein, mFc-His Tag [orb689369]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 60 kDa after removal of the signal peptide.
Mammalian
100 μg, 10 μg, 50 μg Biotinylated Human CD47 [orb2994144]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 42.7 KDa. Observed: 60-70 KDa, reducing conditions
20 μg, 100 μgMouse CD47 [orb2994347]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 42.8 KDa. Observed: 60-90 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg
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Human CD47 (orb2994047)
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