Human CD47 Protein, mFc-His Tag

• Catalog Number: orb689369
• Category: Proteins
• Description: Recombinant human CD47 protein with C-terminal mouse Fc and 6×His tag
• Target: CD47
• Tag: C-Mouse Fc and 6×His Tag
• Reactivity: Human
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: Q08722
• MW: The protein has a predicted molecular mass of 60 kDa after removal of the signal peptide.
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Alternative names: CD47;MER6;IAP;OA3
• Research Area: Epigenetics
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human CD47, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD47, mFc-His tagged protein (orb689369) can bind its native ligand Human SIRPα, hFc-His tagged protein orb689370 in a linear range of 3.3-26.37 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD47, mFc-His tagged protein (orb689369) can bind Anti-CD47 (Neutralizing antibody clone Hu5F9-G4) orb689306 in a linear range of 0.061-1.606 ng/ml.

ELISA plate pre-coated by 1 μg/ml (100 μL/well) Human CD47, mFc-His tagged protein (orb689369) can bind Anti-CD47 Neutralizing antibody orb1173650 in a linear range of 3.2-80.0 ng/ml.