Recombinant Human CD40L Protein

SKU: orb2994033

Description

Recombinant Human CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Glu108-Leu261 is expressed with a 6His tag at the N-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Human CD40-Fc(Cat#orb2994010) on Protein A Biosensor, can bind Human CD40L-His(Cat#orb2994033) with an affinity constant of 2.74 nM as determined in BLI assay. Loaded Anti-Human CD40L mAb-Fc(Cat#orb2993151) on Protein A Biosensor, can bind Human CD40L-His(Cat#orb2994033) with an affinity constant of -3 nM as determined in BLI assay.
• Tag: N-6His
• Molecular Weight: Predicted: 19.1 KDa. Observed: 19-22 KDa, reducing conditions
• Expression Region: Glu108-Leu261
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, 1mM EDTA, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP

Quick Database Links

UniProt

P29965