Recombinant Human CD40L Protein

SKU: orb2995248

Description

Recombinant Human CD40 Ligand is produced by our E.coli expression system and the target gene encoding Met113-Leu261 is expressed.

Research Area

Immunology & Inflammation

Key Properties

• Expression System: E.coli
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human CD40 (C-Fc)(Cat#orb2994010) at 2μg/ml (100 μl/well) can bind Recombinant Human CD40L(Cat#orb2995248)*.*: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human CD40L(Cat#orb2995248) is 1-10 ng/ml. (QC verified). BLI: Loaded Human CD40-His (Cat#orb2995146) on HIS1K Biosensor, can bind Human CD40L (Cat#orb2995248) with an affinity constant of 2.43 nM as determined in BLI assay. (Regularly tested) Loaded Anti-Human CD40L mAb-Fc(Cat#orb2993151) on Protein A Biosensor, can bind Human CD40L(Cat#orb2995248) with an affinity constant of 0.05 nM as determined in BLI assay.
• Tag: Tag free
• Expression Region: Met113-Leu261
• Endotoxins: ＜1EU/μg as determined by LAL test.
• MW: Predicted: 16.2 kDa. Observed: 15 kDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP

Quick Database Links

UniProt

P29965

Huey-Liang Kuo, Chiu-Ching Huang, Tze-Yi Lin, Ching-Yuang Lin IL-17 and CD40 ligand synergistically stimulate the chronicity of diabetic nephropathy Nephrology Dialysis Transplantation, 33, 248 (2018)

PubMed: 28339909

Reactivity

Human