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Human CD40L
SKU: orb2995248
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human CD40 (C-Fc)(Cat#orb2994010) at 2μg/ml (100 μl/well) can bind Recombinant Human CD40L(Cat#orb2995248)*.*: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human CD40L(Cat#orb2995248) is 1-10 ng/ml. (QC verified). BLI: Loaded Human CD40-His (Cat#orb2995146) on HIS1K Biosensor, can bind Human CD40L (Cat#orb2995248) with an affinity constant of 2.43 nM as determined in BLI assay. (Regularly tested) Loaded Anti-Human CD40L mAb-Fc(Cat#orb2993151) on Protein A Biosensor, can bind Human CD40L(Cat#orb2995248) with an affinity constant of 0.05 nM as determined in BLI assay. |
| Tag | Tag free |
| Molecular Weight | Predicted: 16.2 kDa. Observed: 15 kDa, reducing conditions |
| Expression Region | Met113-Leu261 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxins | ＜1EU/μg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP
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Human CD40L (orb2995248)
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