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Description
Research Area
Images & Validation
−
| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | E.Coli |
|---|---|
| Reactivity | Human |
| Tag | N-terminal His Tag |
| Expression Region | 23-126 |
| MW | 13.6 kDa |
| Purity | Greater than 90% as determined by SDS-PAGE |
Storage & Handling
−| Storage | The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle. |
|---|---|
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human CD3D Protein, His Tag and Human CD3E Protein, hFc Tag [orb1743385]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 10.4 and 37.9 kDa after removal of the signal peptide. The apparent molecular mass of CD3D-His and CD3E-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μgRecombinant human CD3E protein, His [orb1516834]
>95% as determined by SDS-PAGE
34 kDa
100 μg, 500 μg, 20 μgCynomolgus CD3D and CD3E Heterodimer Protein, His Tag and hFc Tag [orb2321261]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 10.9 and 37.0 kDa after removal of the signal peptide.
Mammalian
10 μg, 50 μg, 100 μgHuman CD3E Protein, His Tag [orb2321344]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 13.1 kDa after removal of the signal peptide.
Mammalian
10 μg, 50 μg, 100 μgRecombinant Human CD3E Protein, N-His [orb2963003]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
14.32 kDa
1 mg, 50 μg, 100 μg

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