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Description
Research Area
Images & Validation
−Key Properties
−| Source | Mammalian |
|---|---|
| Expression System | HEK293 |
| Target | CD3E |
| Reactivity | Mouse |
| Tag | C-10×His tag |
| Expression Region | CD3E(Asp23-Asp126) 10×His tag |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
| MW | The protein has a predicted molecular mass of 13.1 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 10.4 and 37.9 kDa after removal of the signal peptide. The apparent molecular mass of CD3D-His and CD3E-hFc is approximately 35-55 kDa due to glycosylation.
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The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 10.9 and 37.0 kDa after removal of the signal peptide.
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10 μg, 50 μg, 100 μgRecombinant Human CD3E Protein, N-His [orb2963003]
ELISA, SDS-PAGE, WB
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