Recombinant Human BAFF Protein

SKU: orb2994098

Description

Recombinant Human TNF Ligand Superfamily Member 13B is produced by our Mammalian expression system and the target gene encoding Ala134-Leu285 is expressed with a human IgG1 Fc tag at the N-terminus.

Research Area

Immunology & Inflammation

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human BCMA-His-Flag(Cat#orb2993882) at 1μg/ml (100 μl/well)can bind Human BAFF-Fc(Cat#orb2994098).The ED50 of Human BAFF-Fc(Cat#orb2994098) is 13.62 ng/ml.. BLI: Loaded Human BCMA-His(Cat#orb2993882) on HIS1K Biosensor, can bind Human BAFF-Fc(Cat#orb2994098) with an affinity constant of 0.63 nM as determined in BLI assay. Loaded Human BCMA-His(Cat#orb2994719) on HIS1K Biosensor, can bind Human BAFF-Fc(Cat#orb2994098) with an affinity constant of -3 nM as determined in BLI assay. Loaded Human BCMA-mFc(Cat#orb2994062) on AMC Biosensor, can bind Human BAFF-Fc(Cat#orb2994098) with an affinity constant of -3 nM as determined in BLI assay.
• Tag: N-Fc
• Expression Region: Ala134-Leu285
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 43.3 KDa. Observed: 54 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Tris-HCl，6% Trehalose,4% Mannitol,0.05% Tween 80,10mM Methionine,pH 8.55.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1

Quick Database Links

UniProt

Q9Y275