Biotinylated Human BAFF

SKU: orb2993660

Description

Biotinylated Recombinant Human B-cell-activating factor is produced by our Mammalian expression system and the target gene encoding Ala134-Leu285 is expressed with a Avi tag and a Fc tag at the N-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human BCMA-His-Flag(Cat#orb2993882) at 5μg/ml (100 μl/well)can bind Biotinylated Human BAFF-Avi-Fc(Cat#orb2993660). The ED50 of Biotinylated Human BAFF-Avi-Fc(Cat#orb2993660) is 29.46 ng/ml. (Regularly tested) Immobilized Recombinant Human BCMA-His-Flag(Cat#orb2993882) at 5μg/ml (100 μl/well)can bind Biotinylated Human BAFF-Avi-Fc(Cat#orb2993660). The ED50 of Biotinylated Human BAFF-Avi-Fc(Cat#orb2993660) is 29.46 ng/ml.
• Tag: N-Avi-Fc
• Molecular Weight: Predicted: 44.8 KDa. Observed: 50-60 KDa, reducing conditions
• Expression Region: Ala134-Leu285
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Biotin

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tumor necrosis factor ligand superfamily member 13B; TNFSF13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1; CD257; TNFSF20; ZTNF4

Quick Database Links

UniProt

Q9Y275