Recombinant Human B7-H3 Protein

SKU: orb2992921

Description

Recombinant Human B7 Homolog 3(2Ig) is produced by our Mammalian expression system and the target gene encoding Leu29-Pro245 is expressed with a 6His tag at C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Anti-Human B7-H3 mAb (Cat#orb2993104) on Pro-A Biosensor, can bind Recombinant Human B7-H3 (2Ig, C-6His)(Cat#orb2992921) with an affinity constant of 0.24 uM as determined in BLI assay. (Regularly tested)
• Tag: C-6His
• Molecular Weight: Predicted: 24.1 KDa. Observed: 33-45 KDa, reducing conditions
• Expression Region: Leu29-Pro245
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD276; 2Ig-B7-H3; B7-H3; B7 homolog 3; CD276 antigen; CD276 molecule; Costimulatory molecule

Quick Database Links

UniProt

Q5ZPR3