Recombinant Human B7-H3 Protein (Biotin)

SKU: orb2992954

Description

Biotinylated Recombinant Human CD276 antigen is produced by our Mammalian expression system and the target gene encoding Leu29-Thr461 is expressed with a 6His, Avi tag at C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Biotinylated Human B7-H3 (C-6His-Avi) (Cat#orb2992954) at 2μg/ml (100 μl/well) can bind Anti-Human B7-H3 mAb-Fc(Cat#orb2993104). The ED50 of Anti-Human B7-H3 mAb-Fc(Cat#orb2993104) is 11.42 ng/ml. (Regularly tested). BLI: Loaded Anti-Human B7-H3 mAb (Cat#orb2993104) on Pro-A Biosensor, can bind Biotinylated Human B7-H3 (C-6His-Avi) (Cat#orb2992954) with an affinity constant of 2.59 nM as determined in BLI assay. (Regularly tested)
• Tag: C-6His-Avi
• Molecular Weight: Predicted: 49.1 kDa. Observed: 70-95 kDa
• Expression Region: Leu29-Thr461
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD276; B7H3; 4Ig-B7-H3; B7-H3; B7 homolog 3; CD276 antigen; CD276 molecule; Costimulatory molecule

Quick Database Links

UniProt

Q5ZPR3