Recombinant Histone H3, phosphorylated (pSer10) (Nuclear Marker) Antibody

• Catalog Number: orb750917
• Category: Antibodies
• Description: Mouse monoclonal antibody to H3F3
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: rPHH3/6824
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: A synthetic peptide corresponding to (ARK-pS-TGGKAPRKQLc) of Phosphohistone H3 (phospho S10)
• Antibody Type: Recombinant Antibody
• Concentration: 200 μg/ml
• Dilution range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 15kDa
• Target: H3C1|H3C10|H3C11|H3C12|H3C2|H3C3|H3C4|H3C6|H3C7|H3C8
• Entrez: 8350
• UniProt ID: P68431
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-Hist1h3a antibody, anti-HIST1H3B antibody, an
• Note: For research use only
• Application notes: Positive Control: Human tonsil, liver or breast carcinoma.
• Expiration Date: 12 months from date of receipt.