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Recombinant GST Antibody

SKU: orb1463560

Description

Recombinant GST antibody

Images & Validation

Tested ApplicationsDOT, WB
Dilution RangeWB: 1:200
Application Notes
Anti-GST is a his-tagged monoclonal recombinant antibody designed to detect Glutathione-S-Transferase and its conjugates. This antibody has been tested by western blot and dot blot and is intended for use in immunological assays including western blotting, immunofluorescence and fluorescence activated cell sorting (FACS). Fluorochrome conjugated anti-GST can be used for detection of sensitive non-radioactive in situ hybridization probes. The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of GST-conjugated molecules by immunoblotting or immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user.

Key Properties

Antibody TypePrimary Antibody
ClonalityRecombinant
Clone No.GST13
Source/Expression SystemE. coli
PurityAnti-GST (VHH) Antibody is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture. Expect reactivity against GST-labeled probes and proteins.
ConjugationUnconjugated

Storage & Handling

StorageStore at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: 20% (v/v) Glycerol; Buffer: 1X PBS, pH 7.4
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

recombinant anti-GST antibody, Glutathione-S-Transferase antibody

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
DOT
Dot Blot
View Protocol

Available Sizes

Select a size below

50 μg
Free Secondary Antibody (20 ul)0/0

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