Recombinant Cynomolgus TSLP Protein

SKU: orb2993896

Description

Recombinant Cynomolgus Thymic Stromal Lymphopoietin is produced by our E.coli expression system and the target gene encoding Tyr29-Gln159(Glu37Gln) is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: E.coli
• Biological Origin: Cynomolgus
• Biological Activity: ELISA: Immobilized Anti-Human TSLP mAb (Cat#orb2992721) at 2μg/ml (100 μl/well) can bind Cynomolgus TSLP-His (Cat#orb2993896). The ED50 of Cynomolgus TSLP-His (Cat#orb2993896) is 4.48 ng/ml.. BLI: Loaded Anti-Human TSLP mAb-Fc(Cat#orb2992721) on Protein A Biosensor, can bind Cynomolgus TSLP-His(Cat#orb2993896) with an affinity constant of 0.07 nM as determined in BLI assay.
• Tag: C-6His
• Expression Region: Tyr29-Gln159(Glu37Gln)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 16.2 KDa. Observed: 16 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Tris-HCl, 6% Trehalose, 2% Glycine, 50mM NaCl, 0.05% Tween 80, pH 7.5.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Thymic stromal lymphopoietin;Thymic stroma-derived lymphopoietin;Tslp

Quick Database Links

UniProt

XP_005557555.1