Recombinant Cynomolgus TSLP Protein (Biotin)

SKU: orb2992989

Description

Biotinylated Recombinant Cynomolgus Thymic stromal lymphopoietin is produced by our Mammalian expression system and the target gene encoding Tyr29-Gln159 is expressed with a 6His, Avi tag at the C-terminus.

Key Properties

• Expression System: Human cells
• Biological Origin: Cynomolgus
• Biological Activity: ELISA: Immobilized Anti-Human TSLP mAb (Cat#orb2992721) at 1μg/ml (100 μl/well) can bind Biotinylated Cynomolgus TSLP-His-Avi (Cat#orb2992989).The ED50 of Biotinylated Cynomolgus TSLP-His-Avi (Cat#orb2992989) is 0.097 ng/ml. (Regularly tested). BLI: Loaded Biotinylated Cynomolgus TSLP-His-Avi (Cat#orb2992989) on SA Biosensor, can bind Human TSLPR&IL-7RA Heterodimer-His (Cat#orb2993098) with an affinity constant of 2.15 nM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Cynomolgus TSLP-His-Avi (Cat#orb2992989) on SA Biosensor, can bind Anti-Human TSLP mAb (Cat#orb2992721) with an affinity constant of <1 pM as determined in BLI assay. (Regularly tested)
• Tag: C-6His-Avi
• Molecular Weight: Predicted: 17.8 kDa. Observed: (10-13)&(16-29) KDa, reducing conditions
• Expression Region: Tyr29-Gln159
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 95% as determined by SEC-HPLC.(QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Thymic stromal lymphopoietin; Thymic stroma-derived lymphopoietin; TSLP

Quick Database Links

UniProt

XP_005557555.1