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Recombinant Cdc20 Antibody
Catalog Number: orb2635709
| Catalog Number | orb2635709 |
|---|---|
| Category | Antibodies |
| Description | Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested. |
| Species/Host | Mouse |
| Clonality | Recombinant |
| Clone Number | rCDC20/7184 |
| Tested applications | IHC-P |
| Reactivity | Human |
| Isotype | Mouse IgG1, kappa |
| Immunogen | Recombinant human Cdc20 protein was used as the immunogen for the recombinant Cdc20 antibody. |
| Antibody Type | Primary Antibody |
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
| Conjugation | Unconjugated |
| Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Hazard Information | This recombinant Cdc20 antibody is available for research use only. |
| UniProt ID | Q12834 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Note | For research use only |
| Application notes | Optimal dilution of the recombinant Cdc20 antibody should be determined by the researcher. |
| Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human kidney cancer tissue with recombinant Cdc20 antibody (clone rCDC20/7184) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Cdc20 antibody (clone rCDC20/7184) as confirmation of integrity and purity.
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