Recombinant CD79b Antibody

SKU: orb2643032

Description

CD79 (also designated Ig chains, designated CD79B or B29. The B cell antigen receptor complex (BCR) is formed by the association of CD79 with a membrane immunoglobulin, such as IgM or IgD. The membrane immunoglobulins IgM and IgD achieve surface expression and antigen presentation function in response to CD79 association. The cytoplasmic tails of both CD79A and CD79B contain an ITAM (immuno-receptor tyrosine-based activation) motif, which acts to initiate the BCR signaling reactions by binding to and activating tyrosine kinases.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution Range: ELISA (order BSA-free format for coating): 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant CD79b antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: IGB/3170R
• Immunogen: A recombinant human partial protein (amino acids 29-159) was used as the immunogen for this recombinant CD79b antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human tonsil tissue with recombinant CD79b antibody (clone IGB/3170R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant CD79b antibody (clone IGB/3170R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P40259