Recombinant CD3e Antibody / CD3 epsilon

• Catalog Number: orb2635619
• Category: Antibodies
• Description: CD3 consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids aa 20-40 from the human protein was used as the immunogen for the recombinant CD3e antibody.
• UniProt ID: P07766
• Tested applications: ELISA, IHC-P, WB
• Dilution range: ELISA: 2-4ug/ml (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the recombinant CD3e antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: C3e/4652R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant CD3e antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human tonsil tissue with recombinant CD3e antibody (clone C3e/4652R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node tissue with recombinant CD3e antibody (clone C3e/4652R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue using recombinant CD3e antibody (clone C3e/4652R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Jurkat cell lysate using recombinant CD3e antibody (clone C3e/4652R). Predicted molecular weight ~23 kDa.