Recombinant CD2 Antibody

• Catalog Number: orb2635607
• Category: Antibodies
• Description: CD2 is mainly present on the surface of mature T cells and NK cell membranes, while B cells are usually not expressed.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: LFA2/3417R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant protein fragment from the N-terminal region was used as the immunogen for the recombinant CD2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant CD2 antibody is available for research use only.
• UniProt ID: P06729
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the recombinant CD2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human Jurkat cell lysate using recombinant CD2 antibody (clone LFA2/3417R). Expected molecular weight ~47 kDa.

IHC staining of FFPE human tonsil tissue with recombinant CD2 antibody (clone LFA2/3417R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant CD2 antibody (clone LFA2/3417R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node tissue with recombinant CD2 antibody (clone LFA2/3417R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue with recombinant CD2 antibody (clone LFA2/3417R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CD2 antibody (clone LFA2/3417R) as confirmation of integrity and purity.