Recombinant c-Myc Antibody

SKU: orb2643588

Description

It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FACS, WB
• Dilution Range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells in 0.1ml,Western blot: 2-4ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant c-Myc antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: MYC2895R
• Immunogen: Amino acids 408-439 were used as the immunogen for the recombinant c-Myc antibody. The specific epitope has been localized to amino acids 410-419 (EQKLISEEDL).
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry testing of permeabilized human K562 cells with recombinant c-Myc antibody (clone MYC2895R); Red=isotype control, Blue= recombinant c-Myc antibody.

SDS-PAGE analysis of purified, BSA-free recombinant c-Myc antibody (clone MYC2895R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P01106