Recombinant Aldehyde Dehydrogenase 1A1 Antibody / ALDH1A1

• Catalog Number: orb2635523
• Category: Antibodies
• Description: ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: ALDH1A1/7011R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids 315-434 was used as the immunogen for the recombinant Aldehyde Dehydrogenase 1A1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Aldehyde Dehydrogenase 1A1 antibody is available for research use only.
• UniProt ID: P00352
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Aldehyde Dehydrogenase 1A1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human brain tissue with recombinant Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/7011R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney tissue with recombinant Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1//7011R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/7011R) as confirmation of integrity and purity.