IL2RA Antibody (Biotin)

• Catalog Number: orb1671471
• Category: Antibodies
• Description: IL2RA Antibody (Biotin)
• Target: IL2RA
• Clonality: Polyclonal
• Conjugation: Biotin
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Purification: sIL-2 Receptor alpha-specific antibody was purified by affinity chromatography and then biotinylated
• Immunogen: Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-2 Receptor alpha. Anti-Human sIL-2 Receptor alpha-specific antibody was purified by affinity chromatography and then biotinylated.
• UniProt ID: P01589
• Tested applications: ELISA, WB
• Application notes: Sandwich ELISA: To detect Human sIL-2 Receptor alpha by sandwich ELISA (using 100μl/well) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human sIL-2 Receptor alpha as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human sIL-2 Receptor alpha. Western Blot To detect Human sIL-2 Receptor alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for Recombinant Human sIL-2 Receptor alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: soluble IL-2 receptor, TAC-antigen, CD25 antigen
• Research Area: Immunology & Inflammation, Infectious Disease & Vi
• Note: For research use only
• NCBI: P01589
• Expiration Date: 12 months from date of receipt.

To detect Human sIL-2 Receptor alpha by sandwich ELISA (using 100 μl/well) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human sIL-2 Receptor alpha as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human sIL-2 Receptor alpha.

To detect Human sIL-2 Receptor alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for Recombinant Human sIL-2 Receptor alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.