VDAC1 Antibody

SKU: orb535101

Description

VDAC1 antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:1,000
• Reactivity: Human
• Application Notes:
  Ready to Use Anti-VDAC/Porin Antibody has been optimized and validated in western blot at 1:1000 dilution. This Anti-VDAC/Porin (RTU) Antibody is sufficient to run 10 western blots. Expect a band approximately 30-33kDa in size corresponding to VDAC/Porin by western blotting in appropriate cell lysate or extract. Although not tested, this antibody is likely functional in ELISA and Immunohistochemistry. Optimal titers for applications should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 175-200 of Human VDAC1/Porin1.
• Target: VDAC1
• Purity: RTU Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 0.01% Bovine Serum Albumin (rAlbumin), 25% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: sufficient to run 10 immunoblot tests
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Loading Control Antibody, VDAC-1 Antibody, POR1 Antibody

Western Blot Results of Ready to Use Rabbit Anti-VDAC/Porin Antibody. Lane 1: Opal Pre-stained Molecular Weight Ladder. Lane 2: HEK293T WC Lysate. Lane 3: HeLa WC Lysate (p/n orb348668). Load: 10 µl. Primary Antibody: Ready to Use Rabbit Anti-VDAC1/Porin Antibody 1:1000 overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP (p/n orb347654) at 1:70000 for 30 min RT. Block: BlockOut Buffer (p/n orb348644). Expect: ~31 kDa.

Quick Database Links

Gene Symbol

VDAC1

UniProt

P21796

RefSeq (Protein):NP_003365.1