Ready-To-Use SMAD3 Antibody

• Catalog Number: orb535103
• Category: Antibodies
• Description: SMAD3 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: SMAD3 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
• Antibody Type: Primary Antibody
• Concentration: sufficient to run 10 immunoblot tests
• Dilution range: WB: 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: RTU Anti-SMAD3 Antibody is directed against human Smad3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against human Smad3 protein corresponding to an internal region surrounding amino acid residue 179. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: P84022
• NCBI: NP_005893
• Storage: Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody,
• Note: For research use only
• Application notes: Ready-To-Use Anti-SMAD3 Antibody has been optimized and validated in western blot using 1:1000 dilution. This Anti-SMAD3 (RTU) Antibody is sufficient to run 10 western blots. Expect a band approximately 48.1 kDa in size corresponding to human Smad3 protein by western blotting in the appropriate tissue or cell lysate or extract. Although not tested, this antibody is likely functional in ELISA, immunoprecipitation, and immunohistochemistry. Optimal titers for these applications should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Rabbit anti-SMAD antibody follows the canonical TGF-β signaling pathway. TGF-β dimers bind to a receptor thereby activating the pathway. The type I receptor then recruits and phosphorylates a receptor regulated SMAD (R-SMAD).i.e. SMAD2 or SMAD3. The R- SMAD then binds to the common SMAD (coSMAD) i.e. SMAD4, and forms a heterodimeric complex. This complex then enters the cell nucleus and acts as a transcription factor.

Western Blot Results of Ready to Use Rabbit Anti-SMAD3 Antibody. Lane 1: Opal Pre-stained Molecular Weight Ladder. Lane 2: HEK293T WC lysate. Lane 3: HeLa WC Lysate (p/n orb348668). Load: 10 µl. Primary Antibody: Ready to Use Rabbit Anti-SMAD3 Antibody 1:1000 overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP (p/n orb347654) at 1:70000 for 30 min RT. Block: BlockOut (p/n orb348644). Expect: 63 kDa.