REA (PHB2) Antibody (C-term)

• Catalog Number: orb1929410
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This REA (PHB2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-226 amino acids from the C-terminal region of human REA (PHB2).
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q99623
• MW: 33296 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB10631
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Prohibitin-2, B-cell receptor-associated protein B
• Note: For research use only
• NCBI: NP_001254629.1, NP_001138303.1

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-PHB2 Antibody (C-term) in 293 cell line lysates (35 ug/lane). PHB2 (arrow) was detected using the purified Pab.

All lanes: Anti-PHB2 (Human C-term) at 1:1000 dilution. Lane 1: 293 whole cell lysate. Lane 2: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.