REA (PHB2) Antibody (C-term)

SKU: orb1929410

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology, Infectious Disease & Virology, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD32853
• Immunogen: This REA (PHB2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-226 amino acids from the C-terminal region of human REA (PHB2). Antigen Region: 197-226 aa.
• Target: PHB2 {ECO:0000312|EMBL:AAH14766.1, ECO:0000312|HGNC:HGNC:30306}
• Molecular Weight: 33296 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Prohibitin-2, B-cell receptor-associated protein BAP37, D-prohibitin, Repressor of estrogen receptor activity, PHB2 {ECO:0000312|EMBL:AAH147661, ECO:0000312|HGNC:HGNC:30306}

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-PHB2 Antibody (C-term) in 293 cell line lysates (35 ug/lane). PHB2 (arrow) was detected using the purified Pab.

All lanes: Anti-PHB2 (Human C-term) at 1:1000 dilution. Lane 1: 293 whole cell lysate. Lane 2: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

PHB2 {ECO:0000312|EMBL:AAH14766.1, ECO:0000312|HGNC:HGNC:30306}

UniProt

Q99623

RefSeq (Protein):NP_001138303.1, NP_001254629.1