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Catalog Number | orb1249570 |
---|---|
Category | Antibodies |
Description | RCBTB2 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC |
Predicted Reactivity | Bovine, Mouse, Porcine, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: CEHFRSSLEDNEDD |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments gave an approx. 30kDa band in Human Liver, Lung and Tonsil lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 60.3kDa according to NP_001259.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Testis and Prostate. Recommended concentration: 5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | RCBTB2 |
UniProt ID | O95199 |
NCBI | NP_001259.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | RCBTB2, regulator of chromosome condensation (RCC1 Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments gave an approx. 30kDa band in Human Liver, Lung and Tonsil lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 60.3kDa according to NP_001259.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Testis and Prostate. Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1249570 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1249570 (5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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