RBX1 Recombinant Rabbit Monoclonal Antibody

SKU: orb1499278

Description

RBX1 Recombinant Rabbit Monoclonal Antibody

Research Area

Cell Cycle, Cell Signaling, E3 ubiquitin ligases, Epigenetics, SCF Complex, ubiquitin like modifiers

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1:50-100
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: R2D3
• Immunogen: A synthesized peptide derived from human RBX1 (60-108/108aa)
• Target: RBX1
• Molecular Weight: 12 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

RBX1_HUMAN; E3 ubiquitin-protein ligase RBX1; EC:2.3.2.27; EC:2.3.2.32; RNF75; ROC1; E3 ubiquitin-protein transferase RBX1; Protein ZYP; RING finger protein 75; RING-box protein 1(Rbx1); Regulator of cullins 1(ROC1); E3 ubiquitin-protein ligase RBX1, N-terminally processed; E3 ubiquitin-protein transferase RBX1, N-terminally processed;

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: HepG2, 3: HEK-293, 4: NIH/3T3, 5: PC-12, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 12 kDa, Observed MW: 12 kDa.

Tissue: Human bladder cancer, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high, pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:500, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue).

Tissue: Rat stomach, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:500, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499278.

Quick Database Links

Gene Symbol

RBX1

UniProt

P62877