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Item 1 of 3
RBX1 Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1499278
| Catalog Number | orb1499278 |
|---|---|
| Category | Antibodies |
| Description | RBX1 Recombinant Rabbit Monoclonal Antibody |
| Target | RBX1 |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide derived from human RBX1 |
| UniProt ID | P62877 |
| MW | 12 kDa |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-100, IF=1:50-100 |
| Antibody Type | Recombinant Antibody |
| Clone Number | R2D3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | RBX1_HUMAN; E3 ubiquitin-protein ligase RBX1; EC:2 Read more... |
| Note | For research use only |
Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: HepG2, 3: HEK-293, 4: NIH/3T3, 5: PC-12, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 12 kDa, Observed MW: 12 kDa.
Tissue: Human bladder cancer, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high, pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:500, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue).
Tissue: Rat stomach, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:500, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499278.
