RBP1 Antibody

• Catalog Number: orb1247901
• Category: Antibodies
• Description: RBP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: VEGVVCKQVFKKVQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 16kDa band observed in NIH3T3 and approx 17kDa in U251 cell lysates. The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. (calculated MW of 22.3kDa according to Human NP_002890.2 and 15.8kDa according to Mouse NP_035384.1). Recommended concentration: 0.5-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RBP1
• UniProt ID: P09455
• NCBI: NP_002890.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RBP1, CRBP, RBPC, CRBP1, CRABP-I, retinol binding
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 16kDa band observed in NIH3T3 and approx 17kDa in U251 cell lysates. The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. (calculated MW of 22.3kDa according to Human NP_002890.2 and 15.8kDa according to Mouse NP_035384.1). Recommended concentration: 0.5-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247901 (2 ug/ml) staining of NIH3T3 (A) and (0.5 ug/ml) of U251 (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247901 (5 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247901 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247901 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

orb1247901 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and nuclear staining.