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Catalog Number | orb1417978 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to RBM47. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
Reactivity | Porcine, Rat |
Conjugation | Unconjugated |
UniProt ID | A0AV96 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | RNA-binding protein 47, RNA-binding motif protein Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Sample: A549 Cell (Human) Lysate at 40 ug. A431 Cell (Human) Lysate at 40 ug. Primary: Anti-RBM47 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 57/64 kD. Observed band size: 68 kD.
Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RBM47) Polyclonal Antibody, Unconjugated at 1: 500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Sheep | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |