RBM47 Polyclonal Antibody

• Catalog Number: orb1417978
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RBM47.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Porcine, Rat
• UniProt ID: A0AV96
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: RNA-binding protein 47, RNA-binding motif protein
• Note: For research use only

Sample: A549 Cell (Human) Lysate at 40 ug. A431 Cell (Human) Lysate at 40 ug. Primary: Anti-RBM47 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 57/64 kD. Observed band size: 68 kD.

Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RBM47) Polyclonal Antibody, Unconjugated at 1: 500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.