RBM3 Antibody (Center)

SKU: orb1929297

Description

RBM3 Antibody (Center)

Research Area

Cancer Biology, Cell Biology, Metabolism Research

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 17170 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

RNPL

Western blot analysis of RBM3 antibody (Center) in NIH-3T3 cell line lysates (35 ug/lane). RBM3 (arrow) was detected using the purified Pab.

Western blot analysis of RBM3 (arrow) using rabbit polyclonal RBM3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RBM3 gene.

RBM3 Antibody (Center) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-RBM3 Antibody (Center) at 1:1000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

RBM3 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RBM3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

UniProt

P98179