RBBP7 Antibody

• Catalog Number: orb1260413
• Category: Antibodies
• Description: RBBP7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human RBBP7 (NP_002884.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200ChIP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 48kDa
• Target: RBBP7
• UniProt ID: Q16576
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Histone-binding protein RBBP7, Histone acetyltrans
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200ChIP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using RBBP7 antibody (orb1260413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human gastric cancer using RBBP7 antibody (orb1260413) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using RBBP7 antibody (orb1260413). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293T cells, using RBBP7 antibody (orb1260413) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.