RBBP7 Antibody

SKU: orb1260413

Description

RBBP7 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ChIP, IF, IHC, WB
• Reactivity: Human, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200ChIP: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human RBBP7 (NP_002884.1).
• Target: RBBP7
• Molecular Weight: Observed: 48kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Histone-binding protein RBBP7, Histone acetyltransferase type B subunit 2, Nucleosome-remodeling factor subunit RBAP46, Retinoblastoma-binding protein 7, RBBP-7, Retinoblastoma-binding protein p46, RBBP7, RBAP46

Western blot analysis of extracts of various cell lines, using RBBP7 antibody (orb1260413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human gastric cancer using RBBP7 antibody (orb1260413) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using RBBP7 antibody (orb1260413). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293T cells, using RBBP7 antibody (orb1260413) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Quick Database Links

Gene Symbol

RBBP7

UniProt

Q16576