Rb Rabbit Polyclonal Antibody

SKU: orb6892

Description

Rb Rabbit Polyclonal Antibody

Research Area

Cancer Research, RB Family, Tumor Suppressors

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human P105 RB (51-150/928aa)
• Target: RB1
• Molecular Weight: 102 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RB_HUMAN; Retinoblastoma-associated protein; RB1; p105-Rb; p110-RB1; pRb (Rb); pp110; RB transcriptional corepressor 1; pRb; OSRC; PPP1R130; Retinoblastoma;

Sample: Lane 1: Mouse Thymus tissue lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-Rb (orb6892) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 102 kDa, Observed band size: 135 kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Rb Polyclonal Antibody, Unconjugated (orb6892) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

RB1

UniProt

P06400