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Rat TNFA Protein
Description
Images & Validation
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| Application Notes |
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Key Properties
−| Source | Escherichia Coli |
|---|---|
| Biological Activity | Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is ≤ to 0.2 ng/ml. |
| Protein Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
Storage & Handling
−| Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
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| Form/Appearance | Sterile Filtered colorless liquid. |
| Buffer/Preservatives | TNFA Rat protein solution (1mg/ml) contains Phosphate Buffered Saline (pH 7.4), 10% glycerol and 1mM DTT. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Recombinant Rat TNF alpha Protein [orb3002369]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)
Predicted: 17.4 KDa. Observed: 14 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRat TNF alpha protein [orb754194]
ELISA, WB
Greater than 95% as determined by SDS-PAGE
17.1 kDa
E.Coli
200 μg, 50 μg, 100 μg, 1 mgRat TNF protein [orb707326]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
E. coli
10 μg, 50 μg, 500 μg

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Protocol Information
Rat TNFA Protein (orb610978)
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