Rat Nuclear Factor NF-kappa-B p105 subunit (NF-κB p105) ELISA Kit

• Catalog Number: orb779534
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Kappa B(NFkB).
• Reactivity: Rat
• Concentration: 20 ng/mL
• UniProt ID: Q63369
• Tested applications: ELISA
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.112 ng/mL
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: NF-KB1 ELISA Kit, EBP-1 ELISA Kit, KBF1 ELISA Kit,
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: Please enquire.